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Altaf Wani, PhD



 
  Altaf Wani, PhD
Division Chief of Radiobiology
Professor
1020 Biomedical Research Tower
460 W 12th Avenue
Columbus, OH 43210
Phone: 614-292-9015
Fax: 614-292-9102
Email: wani.2@osu.edu
 

 

Primary Specialty:
Radiology-Radiobiology
Cancer Biology-Mechanistic Studies of DNA Repair
Curriculum Vita

Dr. Wani has been involved in delineating the intricate mechanisms of DNA damage repair for about four decades. His research has utilized a variety of model systems and damaging agents to study several DNA repair pathways. Initial research dealt with revealing the functional features of the individual pathway components in the DNA damage response milieu. Latest efforts have focused on closer inspection and discerning the cross-talk between DNA repair and other associated DNA-templated processes, e.g., chromatin remodeling, transcription, checkpoint activation/deactivation, post-translational protein modifications, protein degradation and documenting the initial status and subsequent fate of DNA damage-modifiable epigenetic histone marks. Having successfully revealed the mechanisms of processing from the perspective of an entire genome or at a single-nucleotide resolution, his research focus has been further sharpened by studies of authenticating DNA repair within the chromatin context. His laboratory was first in the USA to utilize a unique whole-cell based approach for assessing DNA damage and repair within discrete UV radiation-damaged sub-nuclear areas (micropore irradiated foci). For over a decade of the latest multipronged studies, this approach has proven a boon for not only asking questions related to distinct DNA damage responses, but assessing the ‘coming and going’ of a host of individual factors and protein complexes and reveal the spatio-temporal relationships of these proteins with regards to their position and function in different upstream and downstream events of damage response in native environment of the cell. His core research group has extensively published on these facets as well as the interplay of histone modifications, either occurring directly at the site of lesions within chromatin or being deposited as part of newly reconstituted chromatin at the DNA lesion repair sites. As would be expected, his laboratory has deployed a range of state-of-the-art technologies that specifically address his research related goals. Dr. Wani enjoys and takes pride in working on the bench himself and help develop the nitty-gritty protocol details for various old and new experimental procedures, streamlining these for optimum time and resource utilization and the highest quality and quantity of research output. Dr. Wani has devoted himself to exhaustively explore and develop newer paradigms in the realm of molecular oncology, and disseminate the latest and evolving concepts in diverse settings. His overarching goal is to continue work towards the molecular dissection of the processing of genomic lesions and unravel the cross-talk among closely related network of pathways with the ultimate aim of developing intervention strategies for elimination of diseases emanating from inherent instability of the human genome.

Research work in Wani lab has been continuously funded by NIH grants since 1984.

    

Take a photograpic tour of Dr. Wani's Lab

Publication List: 

He, J., Zhu, Q., Wani, G., Sharma, N. and Wani, A.A., VCP/p97 segregase proteolytic processing of CSP in damaged chromatin, J. Biol. Chem., In press (2016).

 
Han, C. Srivastava, A.K., Cui, T., Wang, Q. and Wani, A.A., Differential DNA lesion formation and repair in heterochromatin and euchromatin, Carcinogenesis, Epub ahead of print, (2016).
 
Zhao, R., Cui, T., Han, C., Zhang, X., He, J., Srivastava, A., Yu, J., Wani, A. A., Wang, Q., DDB2 modulates TGF-β signal transduction in human ovarian cancer cells by downregulating NEDD4L,  Nucl. Acids. Res., 43(16): 7839-7849, (2015).
 
Zhu, Q., Sharma, N., He, J., Wani. G. and Wani, A. A., USP7 deubiquitinase promotes ubiquitin-dependent DNA damage signaling by stabilizing RNF168, Cell Cycle, 14(9): 1413-1425, (2015).
 

Zhu, Q., Battu, A., Ray, A., Wani. G., Qian, J., He, J., Wang, Q. and Wani, A. A., Damaged DNA-binding protein down-regulates H3K56Ac through deacetylase 1 and 2, Mut. Res., 776: 16-23, (2015).

Qian, J., Pentz, K., Zhu, Q., Wang, Q., He, J. and Wani. A. A., USP7 modulates UV-induced PCNA monoubiquitination by regulating DNA polymerase eta stability, Oncogene, (2015) 34(36): 4791-4796, (2015).

Han, C., Wani, G., Zhao, R., Qian, Z., Sharma, N., He, J., Zhu, Q., Wang, Q. and Wani., A. A., Cdt2-mediated XPG degradation promotes gap-filling DNA synthesis in nucleotide excision repair, Cell Cycle, 14(7): 1103-1115 (2015).

He, J., Zhu, Q., Wani, G., Sharma, N., Han, C., Qian, J., Pentz, K.,Wang, Q. and Wani. A. A., Deubuquitination by USP7 prevents UV-induced and VCP/P97-regulated XPC proteolysis, J. Biol Chem., 289(39): 27278-89, (2014).

Sharma, N., Zhu, Q., Wani, G., He, J., Wang, Q. and Wani. A. A., USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15, Cell Cycle, 13:1 106-114 (2014).

Zhao, R., Han, C., Eisenhauer, E., Kroger, J., Zhao, W., Yu, J., Karuppaiyah, S., Liu, X., Wani, A. A. and Wang, Q., DNA damage-binding Complex recruits HDAC1 to repress Bcl2 transcription in human ovarian cancer cells, Mol. Cancer Res., 12(3): 370-380, (2014).

Racoma I.O., Meisen, W. H., Wang, Q, Kaur, B., and Wani, A. A., Thymoquinone inhibits autophagy and induces cathepsin-mediated, caspase-independent cell death in glioblastoma cells, PLoS ONE, 8(9): e72882. Doi:10.1371/journal.pone.0072882 (2013).

Altaf A. Wani, Qi-En Wang. Caspase-2 short isoform interacts with membrane-associated cytoskeleton proteins to inhibit apoptosis, PLoS One. 8(7):e67033 (2013).

Ray, A., Milum, K., Battu, A.  and Wani, A.A., NER initiation factors, DDB2 and XPC, regulate radiation response by recruiting ATR and ATM kinases to DNA damage sites, DNA Repair, 12(4): 273-83, (2013).

Wang, Q., Han, C., Zhao, R., Wani, G., Zhu, Q., Gong, L., Battu, A., Racoma, I., Sharma, N., Wani, A. A., p38 MAPK- and Akt-mediated p300 phosphorylation regulates its degradation to facilitate nucleotide excision repair, Nucl. Acids. Res.,. 41 (3): 1722-33, (2013).

Zhu, Q., Wani, G., Sharma, N and Wani, A.A., Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair, DNA Repair, , 11 (12): 942-50, (2012).

Wang, Q., Han, C., Zhang, B., Sabapathy, K. and Wani, A.A., Nucleotide excision repair factor XPC enhances DNA damage-induced apoptosis through down-regulation of anti-apoptotic caspase-2 short isoform, Cancer Res., 72(3): 666-75,  (2012).

Yin, D., Han, C., Chen, L., Wang, Q. Wani, A.A., Gao, J., Germline Stem Cell Gene PIWIL2 Mediates DNA Repair through Relaxation of Chromatin, PLoS ONE., 6(11): e03958,  (2011).

Battu, A., Ray, A.and Wani, A. A., ASF1A and ATM regulate H3K56-mediated cell cycle checkpoint recovery in response to UV irradiation, Nucl. Acids. Res. 39(18): 7931-7945, (2011).

Hassona, M.D.H., Abouelnaga, Z.A., Elnakish, M.T., Alhaj, M., El-Sayed, O., Wani, A.A. and Hassanain, H., Vascular hypertrophy of profilin-1 transgenic mouse leads to cardiac hypertrophy with age., Am. J. Physiol. Heart Circ. Physiol., 57(5); 550-558, (2011).

Wang, Q., Han, C., Milum, K, and Wani, A. A., Stem cell protein Piwil2 modulates chromatin modifications upon cisplatin DNA damage, Mut. Res., 708(1-2): 59-68, (2011).

Wang, Q., Milum, K., Han, C., Huang, Y., Wani, G., Thomale, J. and Wani, A. A., Differential contributory roles of nucleotide excision and homologous recombination repair for enhancing cisplatin sensitivity in human ovarian cancer cells, Mol. Cancer, 10(1): 24 (2011).

Arafa, S.A., Zhu. Q., Shah, Z. I., Barakat, B.M., Wani, G., El-Mahdy, M. A. and Wani, A. A.,Thymoquinone up-regulates PTEN expression and induces apoptosis in doxorubicin-resistant human breast cancer cells, Mut. Res., 706(1-2): 28-35, (2011).

Arab, H. H., Zhu, Q., Wani, G., Ray, A. and Wani, A.A., Defect in CAK recruitment to DNA damage sites and in vivo role of CAK in transcription recovery reveal a link between XP-G/CS and the disassembly of TFIIH, PLoS ONE., 5(6): e11007, (2010).

Zhu, Q. and Wani, A.A., Histone modifications: Critical elements for damage response and chromatin remodeling, J. Cell. Physiol., 223: 283-238 (2010).

Barakat, B. M., Wang, Q., Milum, K., Han, C., Q., Zhao, Q., Yin, D., Wani., G., El-Shaimaa, A. A., El-Mahdy, M. A. and Wani, A. A., DDB 2 enhances the sensitivity of human ovarian cancer cell line to cisplatin by augmenting cellular apoptosis,  Int. J. Cancer., 127(4): 977-988, (2009).

Ye, Y., Xiao, Y., Wang, W., Wang, Q., Yearsley, K., Wani, A., Yan, Q., Gao, J. X., Shetuni, B. S., Barsky, S. H. Inhibition of expression of the chromatin remodeling gene, SNF2L, selectively leads to DNA damage, growth inhibition, and cancer cell death. Mol Cancer Res. 12: 1984-99 (2009).

Arafa, S. A., Zhu, Q.,Barakat, B. M., Zhao, Q., Wani., G., El-Mahdy, M. A. and Wani, A. A., Tangeritin sensitizes cisplatin-resistant human ovarian cancer cells through down-regulation of PI3K/AKT signaling pathway, Cancer Res., 69: 8910-8917, (2009).

Ray, A., Mir, S.N.,  Wani, G., Zhao, Q.,  Zhu, Q., Arab, H., Wang, Q. and Wani, A.A., hSNF5/INI1, a component of human SWI/SNF chromatin remodeling complex, promotes nucleotide excision repair by influencing ATM recruitment and downstream H2AX phosphorylation, Mol. Cell. Biology, 29: 6206-6219, (2009).

Zhao, Q., Wang, Q., Ray, A., Wani, G., Han, C., Milum, K. Wani, A.A., Modulation of DNA damage processing through interaction among components of mammalian SWI/SNF chromatin–remodeling complex and nucleotide excision repair, J. Biol., Chem., 284: 30424-30432, (2009).

Qin, S., Wang, Q., Ray, A., Wani, G., Zhao, Q., Bhaumik, S. and Wani A. A., Sem1p and Ubp6-dependent histone H2B deubiquitination orchestrates telomeric silencing by mediating histone H3 acetylations, Nucl. Acids Res., 37: 1843-53, (2009).

Zhu, Q., Wani, G., Arab, H., El-Mahdy, M.A.,  Ray, A. and Wani, A.A., Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites , DNA Repair, 8: 262-273, (2009).

Zhao, Q., Barakat, B., Qin, S., Ray, A., El-Mahdy, M.A., Wani, G., Arafa, E., Mir, S. N., Wang, Q., Wani, A.A., The p38 mitogen-activated protein kinase augments nucleotide excision repair by mediating DDB2 degradation and chromatin relaxation, J. Biol., Chem., 283: 32553-61, (2008).

El-Mahdy, M.A., Zhu, Q., Wang, Q., Wani, G., Patnaik, S., Zhao, Q., Arafa, E., Barakat, B., Mir, S. N. and Wani, A. A., Naringenin  Protects HaCaT Human Keratinocytes Against UVB-induced Apoptosis and Enhances the Removal of Cyclobutane Pyrimidine Dimers from the Genome, Photochem. Photobiol., 84: 307-316 (2008).

Venkatakrishnan, C.D., Dunsmore, K., Wong, H., Roy, S., Sen, C.K., Wani, A.A., Zweier, J.L. and Ilangovan, G., Heat shock protein 27 regulates p53 transcriptional activity in doxorubicin treated fibroblasts and cardiac H9c2 cells: p21 upregulation and G2/M phase cell cycle arrest, Am. J. Heart & Circ., 294: 1736-1744, (2008).

Wang, Q.,  Prætorius-Ibba, M., Zhu, Q., El-Mahdy, M.A., Wani, G., Zhao, Q., Qin, S. Patnaik, S., and Wani, A.A., UV-induced ubiquitylation-independent Xeroderma pigmentosum group C protein degradation is required for efficient nucleotide excision repair, Nucl. Acids. Res., 35: 5338-50, (2007).

Zhu, Q., Wani, G., Yao, J., Patnaik, S., Wang, Q., El-Mahdy, M.A., Prætorius-Ibba, M. and Wani, A.A., The ubiquitin proteasome system regulates p53-mediated transcription at p21waf1 promoter, Oncogene, 26: 4199-4208, (2007).

Prætorius-Ibba, M., Wang, Q., Wani, G., El-Mahdy, M.A., Zhu, Q., Qin, S. and Wani, A.A., Role of claspin in regulation of nucleotide excision repair factor DDB2, DNA Repair, 6: 578-587 (2007).

Li, J., Wang, Q., Zhu, Q.,  El-Mahdy, M.A., Wani, G., Prætorius-Ibba, M. and Wani, A.A., DNA damage binding component DDB1 participates in nucleotide excision repair through DDB2 DNA binding and cullin 4A ubiquitin ligase activity, Cancer Res., 66:  8590-8597, (2006).

El-Mahdy, M.A., Zhu, Q., Wang, Q., Wani, G., Praetorius-Ibba, M. and Wani, A.A., (2006) Cullin 4A-mediated proteolysis of DDB2 protein at DNA damage sites regulates in vivo lesion recognition by XPC, J. Biol. Chem., 281: 13404-13411 (2006).

Wang, Q.,  Zhu, Q., Wani, G., El-Mahdy, M.A., and Wani, A.A., DNA repair factor XPC is modified by SUMO-1: A novel modification occurs during UV-induced nucleotide excision repair. Nucl. Acids Res., 19, 4023-4034, (2005).

El-Mahdy, M.A, Zhu, Q, Wang, Q., Wani, G and Wani, A.A, Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in p53-null promyelocytic leukemic HL-60 cells. Int. J. Cancer, 117, 409-417, (2005).

Wani, M.A., Zhu, Q., Wang, Q., Chen, J., Wani, G. and Wani, A.A., Dynamics relationship between p53, chromatin remodeling and nucleotide excision repair. Environ. Molec. Mutagen., (in review) (2005).

Zhu, Q., Wani, G., Wang, Q., Snapka, R.M. and Wani, A.A. Deubiquitination by proteasome is coupled to substrate positioning for proteolysis in vivo,  Expt. Cell Res., 307: 436-451, (2005).

Wang, Q., Zhu, Q.,  Wani, M.A., Chen, J., Zhu, Q., Wani, G., El-Mahdy, M.A. and Wani, A.A., Cellular ubiquitination and proteasomal functions positively modulate mammalian nucleotide excision repair, Mol. Carcinog., 42: 53-64, (2005).

Wang, Q., Zhu, Q.,  Wani, G., Chen, J. and Wani, A.A., UV radiation-induced XPC translocation within chromatin is mediated by damaged-DNA binding protein, DDB2, Carcinogenesis, 25: 1033-1043, (2004).

Zhu, Q., Yao, J., Wani, G., Chen, J., Wang, Q., Wani, A.A. The ubiquitin-proteasome pathway is required for the function of the viral VP16 transcriptional activation domain, FEBS Lett., 556:19-25. (2004).

Wang, Q., Zhu, Q.,  Wani, M.A., Wani, G., Chen, J. and Wani, A.A.,) Tumor suppressor p53 dependent recruitment of excision repair factors XPC and TFIIH to DNA damage, DNA Repair, 2: 483-499, (2003).

Wani, M.A., El-Mahdy, M., Hamada, F.M., Wani, G., Zhu, Q.Z., Wang, Q. and Wani, A.A., (2002) Efficient repair of bulky anti-BPDE DNA adducts from non-transcribed strand requires functional p53 but not p21waf1/cip1 and pRB. Mut. Res., 505, 13-25.

Cornwell, D.G., Williams, M.V., Wani, A.A., Wani, G., Shen, E. and Jones, K.H., (2002) Mutagenicity of tocopheryl quinones: Evolutionary advantage of selective accumulation of dietary a-tocopherol, Nutrition and Cancer, 43: 111-118.

Wani, M.A., Wani, G., Yao, J., Zhu, Q.Z. and Wani, A.A., (2002) Human cells deficient in p21 expression exhibit normal nucleotide excision repair of UV induced DNA damage, Carcinogenesis, 23: 403-410

Zhu, Q.Z., Yao, J., Wani, G., Wani, M.A. and Wani, A.A., (2001) Mdm2 mutant defective in binding p300 promotes ubiquitination but not degradation of p53: Evidence for the role of p300 in integrating ubiquitination and proteolysis. J. Biol, Chem. 276: 29695-29701.

Zhu, Q.Z., Wani, G., Wani, M.A. and Wani, A.A., (2001) Regulation of p53 transcriptional activity by hHR23A through p300/CBP, Cancer Res., 61: 64-70.

Zhu, Q.Z., Wani, M.A., El-Mahdy, M., Wani, G. and Wani, A.A., (2000) Modulation of transcriptional activity of p53 by ultraviolet radiation implicates linkage between p53 pathway and DNA repair by damage recognition, Mol. Carcinogenesis, 28: 215-224.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M., Venkatachalam, S. and Wani, A.A., (2000) Enhanced sensitivity to anti-BPDE DNA damage correlates with decreased global genomic repair due to abrogated p53 function in human cells, Cancer Res., 60: 2275-2280.

Zhu, Q.Z., Wani, M.A., El-Mahdy, M. and Wani, A.A., (2000) Decreased DNA repair efficiency by loss or disruption of p53 function preferentially affects removal of cyclobutane pyrimidine dimers from non-transcribed strand and slow repair sites in transcribed strand.  J. Biol Chem., 275: 11492-11497.

El-Mahdy, M., Hamada, F.M., Wani, M.A., Zhu, Q.Z. and Wani, A.A., (2000) p53 degradation by HPV-16 E6 preferentially affects the removal of cyclobutane pyrimidine dimers from non-transcribed strand and sensitizes mammary epithelial cells to UV-irradiation, Mutation. Res., 459: 135-145.

Wani, M.A., Zhu, Q.Z., El-Mahdy, M., and Wani, A.A., (1999) Influence of p53 tumor supressor protein on bias of DNA repair and apoptotic response in human cells, Carcinogenesis, 20: 765-772.

Musarrat, J., Arezina-Wilson, J., Venkatachalam, S., and Wani, A.A. (1997) Repair analysis of promutagenic (+)-anti-BPDE DNA adducts in transcriptionally active sequences of plasmid DNA in Escherichia coli, Biochim. Biophys. Acta, 1351: 203-212.

Venkatachalam, S., Denissenko, M. and Wani, A.A. (1997) Modulation of (±)-anti-BPDE mediated p53 accumulation by inhibitors of protein kinase C and poly (ADP-ribose) polymerase, Oncogene, 14: 801-809.

Musarrat, J., Arezina-Wilson, J. and Wani, A.A. (1996) Localization of O6-alkylguanine transferase in cancer susceptible cells of human female breast, Cancer Lett., 108: 111-118.

Denissenko, M., Venkatachalam, S., Ma, Y. and Wani, A.A. (1996) Single tube immuno-capture and PCR of genotoxin-modified DNA: Application to gene-specific damage analysis, Biotechniques, 21: 187-188.

Musarrat, J., Arezina-Wilson, J. and Wani, A.A. (1996) Prognostic and etiological relevance of 8-hydroxydeoxyguanosine in human breast carcinogenesis, Eur. J. cancer, 32: 1209-1214.

Denissenko, M., Venkatachalam, S., Ma, Y. and Wani, A.A. (1996) Site-specific induction and repair of benzo[a]pyrene diol epoxide DNA damage in human H-ras protooncogene as revealed by restriction cleavage inhibition, Mutat. Res., 363: 27-42.

Musarrat, J., Arezina-Wilson, J. and Wani, A.A. (1995) Repair of Base alkylation damage in targeted restriction endonuclease sequences of plasmid DNA, Biochim. Biophys. Acta, 1263: 201-211.

Venkatachalam, S. Denissenko, M. and Wani, A.A. (1995) DNA repair in human cells: Quantitative assessment of bulky anti-BPDE adducts by non-competitive immunoassays, Carcinogenesis, 16: 2029-2036.

Musarrat, J., Arezina-Wilson, J., Abou-Issa, H. and Wani, A.A. (1995) O6-alkylguanine DNA alkyltransferase activity levels in normal, benign and malignant human female breast, Biochem. Biophys. Res. Commun., 208: 688-696.

Musarrat, J., Arezina, J., Uddin, S. and Wani, A.A. (1995) Induction and processing of promutagenic O4-ethylthymine lesions in discrete sequences within DNA shuttle vectors,  Biochim. Biophys. ACTA, 1260 (3): 276-284.

Wani, A. A., Denissenko, M., Venkatachalam, S., Arezina, J. and Musarrat J. (1994)  Immunoanalysis of genotoxic damage processing, in:  XVI International Cancer Congress Proceedings, Monduzzi  Editore, Intl. Proc. Div.,  Bologna, Italy, Vol  4, pp. 2853-2857.

Venkatachalam, S. and Wani, A.A. (1994) Differential Recognition of Stereochemically Defined Based Adducts by Antibodies Against anti-benzo[a]pyrene Diol-Epoxide-Modified DNA.   Carcinogenesis, 15: 565-572.

Denissenko, M.F., Venkatachalam, S., Yamasaki, E.Y. and Wani, A.A.  (1994)  Assessment of DNA damage and repair in specific genomic regions by quantitative immuno-coupled PCR.  Nucleic Acids Research, 22: 2351-2359.

Musarrat, J. and Wani, A.A. (1994) Quantitative Immunoanalysis of Promutagenic 8-Hydroxy-2'-Deoxyguanosine in Oxidized DNA.  Carcinogenesis, 15: 2037-2043.

Venkatachalam, S., Denissenko, M.F., Alvi, N. and Wani, A.A. (1993) Rapid Activation of Apoptosis in Human Promyelocytic Leukemic Cells by (±)-anti-Benzo[a]Pyrene Diol Epoxide Induced DNA Damage. Biochem. Biophys. Res. Commun. 197: 722-729.

Wani, G., Wani, A.A. and D'Ambrosio, S.M. (1993)  Cell Type-Specific Expression of the O6-Alkylguanine-DNA Alkyltransferase Gene in Normal Human Liver Tissues as Revealed by In Situ Hybridization,  Carcinogenesis, 14: 737-741.

Wani, G., Wani, A.A. and D'Ambrosio, S.M. (1992) In Situ Hybridization of Human Kidney Tissue Reveals Cell Type Specific Expression of the O6-Methylguanine-DNA Methyltransferase Gene,  Carcinogenesis, 13: 463-468.

Yamasaki, E.F., Salamon, D.P. and Wani, A.A. (1992) Mutational Activation of H-ras Oncogene Transformability by Alkylnitrosourea Induced DNA Damage,  Mutation Research, 266: 241-252.

Wani, A.A. and Arezina, J.M. (1991) Immunoanalysis of ultraviolet radiation induced DNA damage and repair in specific gene segments of plasmid DNA,  Biochim. Biophys. ACTA, 1090: 195?203.

Wani, A.A. and Yamasaki, E.F. (1991) Quantitative analysis of carbodiimide modified DNA and immunoprobing by adduct specific antibodies,  Biochim. Biophys. ACTA, 1088: 259-269.

Wani, A.A., Wani, G. and D'Ambrosio, S.M. (1990) Repair of O4-alkylthymine damage in human cells.  In: DNA Damage and Repair in Human Tissues (B.M. Sutherland and A. Woodhead, eds.) Plenum Press, New York, pp. 417-435.  (Refereed)

D'Ambrosio, S.M., Wani, G., Samuel, M., Gibson-D'Ambrosio, R.E. and Wani, A.A. (1990) Repair of O6-methylguanine damage in normal human tissues.   In: DNA Damage and Repair in Human Tissues  (B.M. Sutherland and A. Woodhead, eds.) Plenum Press, New York, pp. 397-416.  (Refereed)

D'Ambrosio, S.M. and Wani, A.A. (1990) Repair of O-alkylation damage in human cells.  In: Transformation of Human Diploid Fibroblast:  Molecular and Genetic Mechanisms  (G. Milo and B.C. Casto, eds.), CRC Press, Inc., Boca Raton, FL, pp. 79-97. (Refereed)

Trewyn, R.W., Yamasaki, E.F. and Wani, A.A. (1990) Solid phase immunoassay for determining the inosine content in transfer RNA.   In: Chromatography and Modification of Nucleosides (C.W. Gehrke and K.C. Kuo, eds.), Elsevier, New York, NY, 45B, pp. 125-142.  (Refereed)

Wani, A.A., Wani, G. and D'Ambrosio, S.M. (1990) A Human DNA Repair Activity Specific for O4?Ethylthymine: Identification and Partial Characterization,  Carcinogenesis, 11: 1419-1424.

Wani, A.A., Sullivan, J.K. and Lebowitz, J. (1989) Immunoassays for Carbodiimide Modified DNA-Detection of Unpairing Transitions in Supercoiled ColE1 DNA,  Nucl. Acids Res., 17: 9957?9977.

Wani, G., Wani, A.A., Gibson-D'Ambrosio, R.E., Samuel, M., Lowder, E. and D'Ambrosio, S.M. (1989) Absence of DNA Damage-Mediated Induction of Human Methyltransferase Specific for Precarcinogenic O6-Methylguanine,  Teratogen. Carcinogen. Mutagen., 9: 259-272.

Mori, T., Wani, A.A., D'Ambrosio, S.M., Chang, G.C. and Trosko, J.E. (1989) In Situ Pyrimidine Dimer Determination by Laser Cytometry, Photochem. Photobiol., 49: 523-526.

Wani, A.A. and D'Ambrosio, S.M. (1987) Immunological Quantitation of O4?Ethylthymidine in Alkylated DNA: Repair of Minor Miscoding Base in Human Cells,  Carcinogenesis, 8: 1137?1144.

Wani, A.A., D'Ambrosio, S.M. and Alvi, N.K. (1987) Quantitation of Pyrimidine Dimers by Immunoslot Blot Following Sublethal UV Irradiation of Human Cells, Photochem. Photobiol., 46: 477?482.

Duran, H.L. and Wani, A.A. (1987) Site Specific Gap?Misrepair Mutagenesis by O4?Ethylthymine, Biochim. Biophys. Acta, 908: 60?69.

D'Ambrosio, S.M., Samuel, Mervyn J., Dutta?Choudhury, T.A. and Wani, A.A. (1987)  O6?Methylguanine DNA Methyl Transferase in Human Fetal Tissues: Fetal and Maternal Factors, Cancer Res., 47: 51?55.

Wani, A.A. and D'Ambrosio, S.M. (1986) Specific DNA alkylation damage and its repair in carcinogen treated rat liver and brain, Arch. Biochem. Biophys., 246: 690?698.

Wani, A.A., Wani, G. and D'Ambrosio, S.M. (1985) Repair of DNA O?alkylation damage by various human organs, Biochem. Biophys. Res. Commun., 133: 589?597.

Wani, A.A., Gibson?D'Ambrosio, R.E. and D'Ambrosio, S.M. (1984) Immunological detection of O6?ethylguanine in alkylated DNA using poly? and monoclonal antibodies, Carcinogenesis, 5: 1145?1150.

Wani, A.A. Gibson?D'Ambrosio, R.E. and D'Ambrosio, S.M. (1984) Antibodies to UV?irradiated DNA: the monitoring of DNA damage by ELISA and indirect immunofluorescence, Photochem. Photobiol., 40: 465?471.

Wani, A.A., Stephens, R.E., D'Ambrosio, S.M. and Hart, R.W. (1982) A new sequence specific endonuclease from M. radiodurans, Biochem. Biophys. Acta, 697: 178?184.

Wani, A.A. and Hart, R.W. (1981) PISUM SATIVUM ENDONUCLEASE:  Further studies on substrate specificity and possible use as biochemical tool, Biochem. Biophys. Acta,  655: 396?406.

Wani, A.A., Rizvi, R.Y. and Hadi, S.M. (1981) Degradation of alkylated DNA by the single?stranded DNA specific endonuclease of pea seeds, Ind. J. Exptl. Biol., 19(3): 206?211.

Wani, A.A. (1980) DNA damage and its accumulation with age.   In: Biological Mechanisms of Aging  (R.T. Schimke, ed.) pp. 211?225. (Refereed)

D'Ambrosio, S.M.,  Daniel, B., Brash, D., Gibson, R.E., Joyce, N., Mhaskar, M., Ortman, J.,  Schmidt, K., Wani, A.A., Witiak, D. and Hart, R.W.  (1980) Recognition of DNA damage in vitro and in vivo.  In:  Polycyclic Aromatic Hydrocarbons, (P. Jones, ed.) Ann Arbor Science Publishers Inc.

D'Ambrosio, S.M., Daniel, F.B., Brash, D.E., Gibson, R.E., Joyce, N.I., Kim, W.I., Mhaskar, D., Ortman,J., Schmidt, K., Wani, A.A., Witiak, D.T. and Hart, R.W. (1979) Recognition of DNA damage in vitro and in vivo.  In:  Polynuclear Aromatic Hydrocarbons: Chemistry and Biological Effects  (A. Bjorseth and A.J. Dennis eds.) Battelle Press, Columbus, Ohio, 4, pp. 523?542.

Wani, A.A. and Hadi, S.M. (1979) Purification and properties of an endonuclease from germinating pea seeds specific for single stranded DNA, Arch. Biochem. Biophys., 196: No. 1, 138?146.

Wani, A.A., Hadi, S.M. and Ahmad, N.S. (1978) Effect of dimethyl sulfate upon the secondary structure of DNA, Experientia, 34: No. 3, 392?394.

Hadi, S.M., Ahmad, N.S. and Wani, A.A. (1978) Deoxyribonuclease activity in larvae of army worm (Spodoptera litura), Insect Biochemistry, 8: No. 4, 297?301.

Wani, A.A. and Hadi, S.M. (1976) A nuclease specific for depurinated DNA in germinating pea (Pisum sativum) seeds, Ind. J. Biochem. Biophys., 13: 307-312.